经典名方泽泻汤薄层色谱鉴别研究

Study on TLC identification method of classical prescription Zexie decoction

摘要:

目的 建立经典名方泽泻汤标准煎液薄层色谱鉴别方法。方法 以23-乙酰泽泻醇C、白术内酯Ⅱ、白术内酯Ⅲ为对照品，采用薄层色谱鉴别法鉴别泽泻汤中泽泻、白术两种药材，并对其耐用性进行考察，确定最佳薄层色谱条件，对15批泽泻汤基准样品进行薄层鉴别。结果 建立了泽泻汤基准样品中泽泻和白术的薄层鉴别方法，且阴性无干扰，15批供试品溶液在与对照品色谱相应位置上显相同颜色的斑点。结论 建立的泽泻汤基准样品薄层色谱鉴别方法简单，专属性强，重复性好，可为泽泻汤基准样品定性鉴别提供方法和依据。

Abstract:

Objective To establish the thin-layer chromatography (TLC) identification methods of Zexie decoction benchmark sample (ZXD).Methods Alisol C 23-acetate, atractylenolide Ⅱ and atractylenolide Ⅲ were used as reference substances, TLC was used to identification Alismatis Rhizoma and Atractylodis Macrocephalae Rhizoma in ZXD, then the durability was investigated. Finally, the optimal thin-layer chromatography conditions were determined, and 15 batches of ZXD were identified simultaneously.Results The TLC identification methods of Alismatis Rhizoma and Atractylodis Macrocephalae Rhizoma in ZXD were established, and there were no negative interference in the test sample solution. The fluorescence spots of the same color were shown in the corresponding position of 15 batches of ZXD sample solution and reference substances.Conclusion The method for identifying Alismatis Rhizoma and Atractylodis Macrocephalae Rhizoma in ZXD has been established.This method is simple, and has strong specificity and good repeatability, which can provide a method and basis for the qualitative identification of ZXD.